COMPONENTS :
Lid : 1 Qty
Trays : 3 Qty
O-Ring : 1 Qty
Base : 1 Qty
Clamp : 1 Qty
Tubing Clamp with Tubing : Qty: 10/bag
Controlled oxygen environments are essential in many cell culture, hypoxia, and oxygen regulation studies. The Modular Incubator Chamber is widely used in research laboratories because it offers a simple, reliable way to control gas conditions without complex equipment.
This guide explains how to open, assemble, gas, seal, and clean the incubator chamber, helping researchers achieve consistent results while protecting their samples and equipment.
What Is a Modular Incubator Chamber?
A modular incubator chamber is a closed system designed to maintain stable oxygen levels including hypoxic or hyperoxic conditions inside a standard laboratory incubator.
It's commonly used for:
- Hypoxia and physioxia studies.
- Cell culture under controlled gas conditions.
- Oxygen-sensitive biological experiments.
Its airtight sealing system helps maintain internal gas composition for extended periods while minimizing evaporation.
How to Open the MIC-101 Chamber Safely?
Proper handling is essential to avoid spills and ensure user safety.
- Hold the chamber and retaining clamp firmly with your left hand, opposite the clamp handle.
- Place your right hand over the clamp handle and open it slowly.
- Fully extend the clamp handle to remove the clamp from the chamber.
- Carefully remove the clamp.
- Using both hands, lift off the lid and trays to prevent accidental spills.
Always handle the lid, base, trays, and clamp with both hands.
How to Reassemble the Modular Incubator Chamber?
- Before reassembly, inspect all sealing surfaces.
Check the O-ring and grooves to ensure they are clean and free of particles.
-Leaks are usually caused by debris or misalignment.
-If needed, adjust the clamp slightly using a 3/8” wrench.
- Place trays with your prepared cell cultures into the base, ensuring proper seating.
- Add humidity by placing a petri dish containing 10–20 ml of sterile water inside the chamber.
- Position the lid onto the base.
- Align the retaining clamp where the lid and base meet and close it slowly, keeping it centered.
The chamber is now ready for gas flushing.
⚠️ Important: Never pressurize the chamber. Both ports must remain open during flushing.
Sealing System Explained
The Modular Incubator Chamber uses a retaining clamp and O-ring sealing system.
As the clamp tightens, it applies inward pressure that compresses the O-ring, creating an airtight seal. The clamp is pre-adjusted at the factory, and only minor adjustments are typically needed over time.
Cleaning and Maintenance Guidelines
Routine cleaning ensures long-term performance.
- Sterilize using 70% ethanol, 5% formalin, or approved sterilizing gas mixtures.
- UV light may be used occasionally, but repeated exposure can degrade polycarbonate.
- Clean the O-ring periodically with 70% ethanol and lint-free wipes.
- Do not apply silicone grease.
- Do not autoclave the chamber.
Proper maintenance helps preserve sealing integrity and experimental consistency.
Features :
- Simple to Use.
- Trustworthy Airtight Sealing: Ensures a dependable and secure closure that reduces contamination.
- Eliminates Evaporation: Prevents multi-well plate “edge effect” evaporation.
- Effortless Cleaning: The seamless, crevice-free interior with rounded corners makes cleaning and decontamination a breeze.
- Minimizes Consumption of Expensive Tri-Gas Mix.
- Uniform Gas Distribution: The cylindrical chamber design guarantees consistent gas distribution.
- Increased Environmental Stability-Zero Lag Time: Once sealed, it maintains consistent gas levels for at least 72 hours, confidently offering extended experiment durations without environmental disturbance.
- Versatile Multipurpose Trays: Accommodates culture dishes, flasks, test tubes, and multi-well plates.
- Space-Efficient Stackable Design: Maximizes storage efficiency.